Characterizing Dolphin Gene Expression with RNA-Seq

In order to examine the effects of chronic exposure to pollutants on the dolphin transcriptome, I am working to characterize the skin transcriptome of long-beaked common dolphins (Delphinus capensis) in the Southern California Bight using high throughput sequencing of RNA (RNA-Seq). I am seeking to understand seasonal variation in gene expression and to determine how accumulated heavy metals influence expression patterns. RNA is extracted from dart biopsies provided by Dr. Nicholas Kellar (NOAA Fisheries, Southwest Fisheries Science Center), converted to cDNA and sequenced on an Illumina Hi-Seq sequencer. Concentrations of RNA are quantified at UC Davis’ CAHFS.

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